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抗泛素抗體Anti-Ubiquitin antibody [Ubi-1]

Description Mouse monoclonal [Ubi-1] to Ubiquitin
Host species Mouse
Specificity Ubi-1 was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. It was one of several
clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that Ubi-
1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of
Alzheimer's disease. Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and
the Pick bodies in Pick's disease. This antibody recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules
or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
Tested applications IHC-P, WB, ELISA, ICC/IF, IHC-Fr
Cross reactivity Reacts with
Mouse, Chicken, Cow, Human, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly （Drosophila melanogaster）
Immunogen Full length native protein （purified） conjugated to KLH by a Glutaraldehyde linker.
Target
Relevance Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins
for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin,
and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently
bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies
are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of
Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated
and can be readily visualized with ubiquitin antibodies.
Cellular localization Cell Membrane, Cytoplasmic and Nuclear
Alternative names FLJ25987 antibody
MGC8385 antibody
Polyubiquitin B antibody
RPS 27A antibody
RPS27A antibody
UBA 52 antibody
UBA 80 antibody
UBA52 antibody
UBA80 antibody
UBB antibody
UBC antibody
UBCEP 1 antibody
UBCEP 2 antibody
UBCEP1 antibody
UBCEP2 antibody
Ubiquitin antibody
ubiquitin B antibody
Properties
Form Liquid
Storage instructions Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

抗泛素抗體Anti-Ubiquitin antibody [Ubi-1] 反應性有其他種屬的：人，小鼠，大鼠等等

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